论文部分内容阅读
目的探讨TRPV1基因敲除后外周痛觉的改变。方法采用甩尾热痛仪和弗莱毛测痛法测量TRPV1基因敲除型及野生型雌性小鼠的热和机械痛阈,并进行比较。结果热刺激后,TRPV1基因敲除型雌性小鼠较野生型雌性小鼠的甩尾潜伏期延长[(3.59±0.65)s vs(2.19±0.24)s,P<0.05],两组间机械痛阈值差异无统计学意义[(1.71±0.57)g vs(2.13±0.81)g,P>0.05]。结论在生理条件下,TRPV1受体介导热刺激痛,与机械刺激痛无明显相关。
Objective To investigate the changes of peripheral pain perception after TRPV1 knockout. Methods The thermal and mechanical pain thresholds of TRPV1 gene knockout and wild type female mice were measured by flick-tachyphus and Fletcher’s method, and compared. Results After heat stimulation, the tail flick latency of TRPV1 knockout female mice was longer than that of wild type mice [(3.59 ± 0.65) s vs (2.19 ± 0.24) s, P <0.05]. The mechanical pain threshold The difference was not statistically significant [(1.71 ± 0.57) g vs (2.13 ± 0.81) g, P> 0.05]. Conclusion Under physiological conditions, TRPV1 receptor mediates heat-induced pain, which is not significantly associated with mechanical stimulation of pain.