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目的：了解有机磷和拟除虫菊酯两类杀虫剂对家蝇线粒体膜脂流动性的影响。方法：采用荧光偏振法，以ＤＰＨ为荧光探剂。结果：６种有机磷化合物和４种拟除虫菊酯与家蝇线粒体膜作用３～６分钟，即可引起膜流动性的明显改变。对膜流动性影响的方式因杀虫剂结构上的差异而有所不同。氯菊酯和倍硫磷、马拉硫磷、敌百虫、氧化乐果可使流动性增加，而氰戊菊酯、氯氰菊酯、溴氰菊酯和辛硫磷、乙酰甲胺磷则可使膜流动性下降。不同杀虫剂对相变温度的影响也存在差异。溴氰菊酯和乙酰甲胺磷、氧化乐果、倍硫磷可使相变温度降低１℃，而氯氰菊酯和马拉硫磷、敌百虫则分别使相变温度增加２、３和３℃，但氯菊酯对相变温度的影响不明显。结论：这两类杀虫剂对生物膜流动性的影响，很可能是其作用机制中的一个关键组分
Objective: To understand the effects of organophosphorus and pyrethroid insecticides on mitochondrial membrane lipid fluidity in housefly. Methods: Fluorescence polarization method and DPH as fluorescent probe. Results: 6 kinds of organophosphorus compounds and 4 kinds of pyrethroids and Musca domestica mitochondrial membrane function 3 ~ 6 minutes, can cause significant changes in membrane fluidity. The way in which membrane fluidity is affected varies depending on the structural differences of the pesticides. Permethrin and fenthion, malathion, trichlorfon, and omethoate increase fluidity, whereas fenvalerate, cypermethrin, deltamethrin and phoxim, acephate, Membrane fluidity decreased. Different pesticides on the phase transition temperature there are differences. Deltamethrin and acephate, omethoate, and fenthion led to a 1 ° C reduction in phase transition temperature, whereas cypermethrin, malathion and trichlorfon increased the phase transition temperature by 2, 3 and 3 ° C, respectively , But the effect of permethrin on the phase transition temperature is not obvious. CONCLUSIONS: The effects of these two classes of insecticides on the fluidity of biomembranes are likely to be a key component of their mechanism of action