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目的 观察不同时点给予异丙酚对内毒素休克大鼠的作用及机制。方法 静脉给予脂多糖(LPS)8 mg·kg~(-1) 复制内毒素休克模型,雄性 Wistar大鼠76只随机分5组:对照组(A组);LPS组(B组);C、D、E组为异丙酚+LPS组,分别于LPS注入前1h、LPS注入即刻、LPS注入后1h,均静注异丙酚5 mg·kg~(-1),继以10 mg·kg~(-1)·h~(-1)持续泵注。观察动物 MAP、PaO_2,pH,血清肿瘤坏死因子(TNF-α)、一氧化氮(NO)浓度,肺组织TNF-α及丙二醛(MDA)含量、髓过氧化物酶(MPO)活性的变化。并比较各组存活率。结果 与B组比较,C、D组可不同程度地逆转MAP、PaO_2 、pH的下降,抑制血清TNF-α、NO水平及肺组织TNF-α、MDA、MPO水平的升高,动物存活率提高(P<0.01或0.05)。E组上述指标改善较C组、D组弱。结论 异丙酚早期给药对内毒素休克起保护效应。
Objective To observe the effect and mechanism of propofol administered at different time points in endotoxic shock rats. Methods Lipopolysaccharide (LPS) 8 mg · kg -1 was used to replicate endotoxic shock model. 76 male Wistar rats were randomly divided into 5 groups: control group (group A), LPS group (group B) D and E groups were treated with propofol + LPS group, propofol 5 mg · kg -1 at 1 hour before LPS injection, immediately after LPS injection, and 1 hour after LPS injection respectively, followed by 10 mg · kg -1 ~ (-1) · h ~ (-1) continuous pump injection. The contents of MAP, PaO_2, pH, serum TNF-α, NO, lung tissue TNF-α and malondialdehyde (MDA) Variety. And compare the survival rate of each group. Results Compared with group B, group C and group D could reverse the decrease of MAP, PaO 2 and pH to some extent, and inhibit the increase of serum TNF-α, NO and the level of TNF-α, MDA and MPO in lung tissue and the increase of animal survival rate (P <0.01 or 0.05). E group than the above indicators to improve the C group, D group is weak. Conclusion Propofol can protect endotoxin shock from early administration.