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目的:了解MTHFR基因677和1298位点多态性与宫颈癌发病的关系。方法:应用PCR扩增技术,其扩增产物经限制性核酸内切酶HinfI和MboⅡ酶消化后进行琼脂糖凝胶电泳,分析MTHFR基因型的分布情况。结果:宫颈癌患者157例,CIN患者38例,异常对照组183例,正常对照组199例,发现MTHFR基因的677位点多态性分布差异并无统计学意义(P>0.05),1 298位点多态性的分布在各组间差异有统计学意义(P<0.05)。结论:由于地理环境、种族和生活条件的不同,相同的基因突变在不同的人群中所起的作用可能不同。
Objective: To investigate the relationship between polymorphisms of MTHFR gene 677 and 1298 and the incidence of cervical cancer. Methods: The PCR products were digested with restriction endonucleases HinfI and MboⅡ and then analyzed by agarose gel electrophoresis. The distribution of MTHFR genotypes was analyzed. Results: There were 157 cases of cervical cancer, 38 cases of CIN, 183 cases of abnormal control group and 199 cases of normal control group. There was no significant difference in the polymorphism distribution of MTHFR 677 locus (P> 0.05) The distribution of polymorphism in each group was statistically significant (P <0.05). CONCLUSIONS: The same genetic mutations may play different roles in different populations due to geographical differences, races, and living conditions.