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我们应用ａｃｔｉｎ（平滑肌型）单克隆抗体对３０例乳腺疾病及肿瘤进行标记，以观察ａｃｔｉｎ的变化，探讨其在早期恶变诊断中的生物学意义。观察可见：乳腺增生病、乳腺纤维腺瘤的肌上皮完整，但肌上皮细胞增生，ａｃｔｉｎ为阳性。乳腺单纯癌、浸润性导管癌、管状腺癌等恶性浸润性肿瘤，ａｃｔｉｎ表达为阴性，说明平滑肌型ａｃｔｉｎ缺如，１例平滑肌肉瘤ａｃｔｉｎ阳性，可与纤维肉瘤、神经纤维肉瘤鉴别。乳腺导管原位癌早期浸润处ａｃｔｉｎ阴性，表明肌上皮细胞消失。乳腺增生病早期恶变处、小叶原位癌早期浸润部分，ａｃｔｉｎ呈阴性反应。因此，ａｃｔｉｎ蛋白的表达，可作为判断乳腺增生病早期恶变、导管原位癌及小叶原位癌早期浸润的重要辅助指标。
We applied actin (smooth muscle) monoclonal antibody to mark 30 cases of breast diseases and tumors to observe the changes of actin and explore its biological significance in early malignant diagnosis. Observed: mammary gland hyperplasia, breast fibroadenoma myoepithelial integrity, but the proliferation of myoepithelial cells, actin positive. In malignant infiltrating tumors such as simple breast cancer, invasive ductal carcinoma, and tubular adenocarcinoma, the expression of actin was negative, indicating that smooth muscle actin was absent, and 1 case of leiomyosarcoma was actin positive, which could be differentiated from fibrosarcoma and fibrosarcoma. The presence of actin-negative invasive sites in ductal carcinoma in situ of the breast shows that myoepithelial cells have disappeared. Early malignant transformation of breast hyperplasia and early infiltration of lobular carcinoma in situ resulted in negative actin expression. Therefore, the expression of actin protein can be used as an important auxiliary indicator to judge the early malignant transformation of mammary gland hyperplasia, ductal carcinoma in situ and early infiltration of lobular carcinoma in situ.