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为探讨蜂毒肽对心血管系统的作用机理，利用离体大鼠主动脉环收缩实验及血管平滑肌细胞４５ Ｃａ 内流定量测定方法，观察了蜂毒肽对血管平滑肌的影响。结果发现：蜂毒肽不影响内皮完整主动脉环由苯肾上腺素引起的快速收缩，但可浓度依赖地抑制持续收缩；蜂毒肽对去内皮主动脉环由苯肾上腺素引起的收缩无作用；蜂毒肽对内皮完整主动脉环未能产生收缩反应，但可使去内皮动脉环产生显著收缩和４５ Ｃａ 内流增加，卡托普利和苄普地尔能部分拮抗这一作用。结果提示，蜂毒肽具有内皮依赖性舒张血管平滑肌的作用，肾素释放与 Ｎａ ＋ － Ｃａ２ ＋ 交换途径可能参与蜂毒肽使去内皮血管收缩。
To investigate the mechanism of action of melittin on the cardiovascular system, the effects of melittin on vascular smooth muscle were observed by contractile test of isolated aortic rings of rats and quantitative evaluation of 45 Ca influx in vascular smooth muscle cells. The results showed that melittin did not affect the fast contraction induced by phenylephrine in the intact aortic rings, but inhibited the contraction in a concentration-dependent manner. The melittin had no effect on the contraction induced by phenylephrine in the aortic rings of the endothelium. Melittin failed to produce a contractile response to the intact aortic rings of the endothelium, but produced significant contraction of the endothelium and increased 45 Ca influx, which was partially antagonized by captopril and bepridil. The results suggest that melittin can relax the vascular smooth muscle cells in an endothelium - dependent manner. The renin release and Na + - Ca2 + exchange pathway may participate in the denervation of endothelial vasoconstriction by melittin.