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目的评价芍药舒筋片保护C57黑鼠软骨组织及其抗炎、镇痛作用,为其临床应用提供实验依据。方法将70只黑鼠随机分为空白对照组,模型对照组,阳性对照组,芍药舒筋片高、中、低剂量组。通过跑步机运动负荷成模后,灌胃给药4周,然后取膝关节进行病理形态学观察。采用小鼠耳郭肿胀、大鼠足跖肿胀模型,分为空白对照组,阳性对照组,芍药舒筋片高、中、低剂量组,分别干预5天后观察芍药舒筋片的抗炎作用;采用小鼠热板法和扭体法,分为空白对照组,阳性对照组,芍药舒筋片高、中、低剂量组,分别采取相应的干预措施,研究芍药舒筋片的镇痛作用。结果芍药舒筋片可明显减轻C57黑鼠膝骨关节炎关节软骨损伤程度;Mankin评分提示,阳性对照和治疗药组与模型组比较差异均有统计学意义(P<0.01),且芍药舒筋片高、中剂量组作用优于阳性药维固力组。芍药舒筋片能降低巴豆油致小鼠外耳郭的肿胀度和肿胀率,阳性对照组和给药组与空白组比较差异均有统计学意义(P<0.05,P<0.01);对鸡蛋清致大鼠足跖的肿胀度和肿胀率亦有明显的减轻作用,阳性对照组和高、中剂量给药组与空白组比较差异均有统计学意义(P<0.05,P<0.01);阳性对照组和高、中剂量给药组与空白组小鼠痛阈值比较,差异均有统计学意义(P<0.01);阳性对照组和给药组与空白组小鼠扭体次数比较,差异均有统计学意义(P<0.01)。结论芍药舒筋片可一定程度延缓运动负荷导致的C57黑鼠膝骨关节炎关节软骨退变,且有较好的抗炎镇痛作用。
Objective To evaluate the protective effects of Shaoyu Soothing Tablet on C57 black rat cartilage tissue and its anti-inflammatory and analgesic effects, and to provide experimental evidence for its clinical application. Methods Seventy black rats were randomly divided into blank control group, model control group, positive control group, Shaoyu Soothing Tablets high, medium and low dose groups. Treadmill exercise load through the mold, gavage for 4 weeks, and then take the knee for pathological observation. The mouse ear swelling, rat paw edema model was divided into blank control group, positive control group, peony Shujin tablets high, medium and low dose groups, respectively, after 5 days of intervention to observe the anti-inflammatory effect of Shaoyu Soothing tablets; The mice were divided into blank control group, positive control group, high and medium dosage and low dosage of Paeoniflorin Tablet by mouse hot plate method and writhing method, and the corresponding intervention measures were respectively taken to study the analgesic effect of Shaoyu Soothing tablets. Results Paeoniflorin tablet can significantly reduce the degree of articular cartilage injury of knee osteoarthritis in C57 black rats. The Mankin score suggests that there is significant difference between the positive control group and the therapeutic group and the model group (P <0.01) Tablet high dose group is better than the positive drug force group. Paeoniflorin tablets can reduce the degree of swelling and swelling rate of croton oil-induced auricle in mice. There was significant difference between the positive control group and the administration group and the blank group (P <0.05, P <0.01) The degree of swelling and swelling rate of rats’ feet and so on were also significantly relieved. There was significant difference between the positive control group and the high and medium dose administration groups and the blank group (P <0.05, P <0.01); the positive The pain thresholds in the control group and the high dose and middle dose groups were significantly different from those in the blank group (P <0.01), while those in the positive control group and the blank group were significantly lower than those in the blank group There was statistical significance (P <0.01). Conclusion Shaoyu Shujin tablet can decelerate the degeneration of articular cartilage of knee osteoarthritis caused by exercise load to a certain extent and has good anti-inflammatory and analgesic effects.