论文部分内容阅读
以酸性土壤耐受能力差异明显的2个大豆品种丹波黑大豆和云南小黑豆为材料,分别在pH 4.55的黄壤、pH5.60的红壤和pH 7.14的正常土壤上盆栽种植,于出苗后50 d观察生长状况并测定根和叶的主要生理指标,研究大豆对酸性土壤胁迫的耐受生理机理。结果表明:在弱酸和强酸性土壤上丹波黑大豆都能形成根瘤、生长良好,而云南小黑豆则不能形成根瘤、长势差。2个黑豆根中产生的活性氧簇(ROS)主要通过提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性来清除,其中云南小黑豆根中这些酶活性明显提高,受酸性土壤胁迫影响较大;2个黑豆叶中则产生大量可溶性糖、脯氨酸(Pro)、丙二醛(MDA)、蛋白质羰基(PC)和过氧化氢(H2O2)来维持较低水平的渗透压与膜脂过氧化水平以提高对酸性土壤胁迫的耐受能力。结果证明丹波黑大豆耐酸性土壤胁迫能力明显强于云南小黑豆。
Two soybean cultivars, Danbo black soybean and Yunnan black soybean, which had obvious difference in acid soil tolerance, were planted in pots with pH 4.55, red soil pH5.60 and normal soil pH 7.14, respectively. After 50 days Growth status was observed and the main physiological indexes of roots and leaves were determined to study the physiological mechanism of soybean tolerance to acid soil stress. The results showed that the root tuber could form nodules on both weak acid and strong acid soils, and the growth was good while that of Yunnan black beans could not form nodules. The reactive oxygen species (ROS) produced in the roots of two black beans were mainly eliminated by increasing the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) The activities of these enzymes were significantly increased, which were greatly affected by acid soil stress. A large amount of soluble sugar, proline (Pro), malondialdehyde (MDA), protein carbonyl (PC) and hydrogen peroxide ) To maintain a lower level of osmotic pressure and membrane lipid peroxidation levels to improve tolerance to acid soil stress. The results showed that the acid tolerance of Danbo black soybean was significantly stronger than that of Yunnan black bean.