爬行卫矛下胚轴高频离体再生体系的建立(英文)

• 作 者: 尚爱芹 孙振元 赵梁军
• 机 构: 中国农业大学农业与生物技术学院,河北农业大学园艺学院,中国林业科学研究院林业研究所国家林业局林木培育重点实验室,
• 出 处: 林业科学
• 发表日期: 2009年02期
• 关键词: 卫矛 爬行卫矛 直接器官再生 组织学观察 观赏植物 离体培养 器官再生 shoot 离体再生 下胚轴

In this paper,a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei var.radicans.Some factors that influenced shoot regeneration such as different combinations of plant growth regulators,types of medium and inoculation ways were studied in order to establish an efficient plant regeneration for transformation.The results showed that hypocotyl explants were horizontally cultured on a basic medium composed of MS medium supplemented with 0.5 mg·L-1 BAP and 0.01 mg·L-1 NAA for induction and development of adventitious shoots.Ninety-four percent of regeneration frequency and 5.1 shoots per explants were obtained after 30 days of culture.Regenerated shoots proliferated efficiently on a shoot multiplication medium consisting of MS medium containing 1.0 mg·L-1 BAP and 0.1 mg·L-1 NAA.Microshoots were rooted on a rooting medium made up of MS medium enriched with 0.5 mg·L-1 IBA and 0.5 mg·L-1 IAA.After hardening,90% of plants were successfully established under greenhouse conditions.Histological observation revealed that shoot primordium originated from subepidermal cells of hypocotyl explants and directly developed into adventitious shoots without callus formation.

In this paper, a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei var. Radicans. Some factors that influenced shoot regeneration such as different combinations of plant growth regulators, types of medium and inoculation ways were studied in order to establish an efficient plant regeneration for transformation. The results showed that hypocotyl explants were cultured in a basic medium composed of MS medium supplemented with 0.5 mg · L-1 BAP and 0.01 mg · L-1 NAA for induction and development of adventitious shoots. Ninety -four percent of regeneration frequency and 5.1 shoots per explants were obtained after 30 days of culture. Regenerated shoots proliferated efficiently on a shoot multiplication medium consisting of MS medium containing 1.0 mg · L-1 BAP and 0.1 mg · L -1 NAA. Microshoots were rooted on a rooting medium made up of MS medium enriched with 0.5 mg · L -1 IBA and 0.5 mg · L -1 IAA. After hardening, 90% of plants were successfully established under greenhouse conditions. Histological observation revealed that shoot primordium originated from subepidermal cells of hypocotyl explants and directly developed into adventitious shoots without callus formation.