论文部分内容阅读
目的:建立太白蓼的高效液相色谱特征图谱,用于其质量控制。方法:采用HPLC法梯度洗脱,检测波长280 nm,以甲醇-乙腈(60∶40)为流动相A,以0.5%甲酸溶液为流动相B,流速1.0 m L·min~(-1),以咖啡酸为参照物计算10批样品共有峰相对保留时间;采用超高效液相色谱串联四极杆飞行时间质谱法(UPLC/Q-TOF-MS法)对太白蓼进行鉴定,并在特征图谱中予以指认。结果:检出8个共有峰,峰面积均大于10 000,通过质谱鉴定确认了其中3个共有峰(绿原酸、咖啡酸、表儿茶素),在质谱鉴定的基础上遴选咖啡酸峰为参照峰,特征峰与参照峰相对保留时间的RSD均小于5%。结论:该特征图谱可用于太白蓼药材鉴别及质量控制,并能区别其类似的混淆品种珠芽蓼、圆穗蓼。
OBJECTIVE: To establish a high performance liquid chromatographic profile of Polygonum orientale for its quality control. Methods: The HPLC method was used for the gradient elution. The detection wavelength was 280 nm. The mobile phase A was methanol - acetonitrile (60:40), the mobile phase B was 0.5% formic acid and the flow rate was 1.0 m L · min -1. The caffeic acid was used as a reference to calculate the relative retention time of common peaks of ten batches of samples. Polygamy was identified by UPLC / Q-TOF-MS with ultra performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry In the be identified. Results: Eight common peaks were detected and their peak areas were all more than 10 000. Three common peaks (chlorogenic acid, caffeic acid and epicatechin) were confirmed by mass spectrometry, and caffeic acid peaks were selected on the basis of mass spectrometry As the reference peak, the RSDs of the relative retention times of the characteristic peak and the reference peak are all less than 5%. Conclusion: This characteristic map can be used for the identification and quality control of Polygonum capitatum, and can distinguish its similar confused varieties Polygonum viviparum and Polygonum orientale.