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[目的]通过观察冠心康颗粒剂对镉中毒大鼠氧化损伤的影响,为研究环境毒邪所致衰老的机制以及中药防护作用,从而为衰老的预防和控制提供理论依据。[方法]选择72只健康Wistar雄性大鼠,随机分为空白组、模型组、西药组、中药低、中、高剂量组,每组12只,每只体重250±50g。除了空白组以外,均给0.05mg/Kg镉溶液,连续2周后停镉,同时西药组给2mL硫酸锌溶液,中药低、中、高剂量组分别给1.5g/(Kg.d)、3g/(Kg.d)、6g/(Kg.d)的冠心康颗粒,连续至60天,实验结束后结果模型组大鼠死亡2只。测定端粒酶(Telomerase)活性、丙二醛(MDA)和超氧化物歧化酶(SOD)含量。[结果]与空白组比较,各组的端粒酶活性和SOD活力明显下降,MDA含量明显增高。(P<0.05)西、中药处理组与模型组比较,端粒酶活性和SOD活力增高,MDA含量降低(P<0.05)。[结论]镉中毒可引起氧化性损伤导致促进衰老,硫酸锌和冠心康颗粒剂可以拮抗镉的毒性,提高抗氧化能力,从而可能延缓衰老。
[Objective] To observe the effect of Guanxinkang Granule on the oxidative damage of cadmium poisoning rats, and provide a theoretical basis for the prevention and control of aging in order to study the mechanism of aging caused by environmental poison and the protective effect of traditional Chinese medicine. [Methods] Seventy - two healthy Wistar male rats were randomly divided into blank group, model group, western medicine group and traditional Chinese medicine low, medium and high dose group, with 12 rats each weighing 250 ± 50g. In addition to the blank group, were given 0.05mg / Kg solution of cadmium, two weeks after the suspension of cadmium, while Western medicine group to 2mL zinc sulfate solution, low, medium and high dose groups were given 1.5g / (Kg.d), 3g / (Kg.d), 6g / (Kg.d) of coronary heart particles, continuous to 60 days after the end of the experiment results in the model group of rats died 2. Telomerase activity, malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. [Results] Compared with the blank group, the telomerase activity and the SOD activity in each group were significantly decreased, MDA content was significantly increased. (P <0.05). Compared with the model group, the activity of telomerase and the activity of SOD increased and the content of MDA decreased (P <0.05). [Conclusion] Cadmium poisoning can cause oxidative damage leading to the promotion of senescence. Zinc sulfate and Guanxinkang granules can antagonize the toxicity of cadmium, improve the anti-oxidative capacity, and thus may delay aging.