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目的:检测子宫内膜癌组织中尿激酶型纤溶酶原激活物(uPA)及组织蛋白酶(Cath-D)的表达并探讨相关性及其临床意义。方法:采用免疫组织化学方法(PV-6000二步法)检测31例子宫内膜癌组织(内膜癌组),17例子宫内膜增生组织(增生组)及10例正常子宫内膜组织(对照组)中uPA及Cath-D的表达,并研究其相关性。结果:1.内膜癌组中uPA和Cath-D的表达均高于增生组及对照组中的表达,差异均有统计学意义(P<0.05);在增生组中的表达与对照组差异无统计学意义(P>0.05)。2.uPA和Cath-D的阳性表达与子宫内膜癌的临床病理分期、组织学分级及肌层浸润深度有关,差异均具有统计学意义(P<0.05)。3.内膜癌组中uPA与Cath-D的表达呈正相关(r=0.673,P<0.05)。结论:uPA和Cath-D在子宫内膜癌发生发展及侵袭转移过程中起着协同作用,Cath-D可诱导产生活化的uPA,促进癌细胞的浸润转移,因此,两者的联合检测可有助于成为判断子宫内膜癌的发展及预后的重要指标。
Objective: To detect the expression of urokinase-type plasminogen activator (uPA) and cathepsin (Cath-D) in endometrial carcinoma and to explore the correlation and its clinical significance. Methods: 31 cases of endometrial carcinoma (endometrial carcinoma), 17 cases of endometrial hyperplasia (hyperplasia) and 10 cases of normal endometrium (endometrial carcinoma) were detected by immunohistochemistry (PV-6000 two-step method) Control group) uPA and Cath-D expression, and to study the correlation. The expression of uPA and Cath-D in endometrial carcinoma group were higher than those in the proliferative and control groups (P <0.05), and the difference between the expression in the proliferative group and the control group No statistical significance (P> 0.05). The positive expression of uPA and Cath-D was related to the clinicopathologic stage, histological grade and the depth of myometrial invasion in endometrial carcinoma (P <0.05). 3. The expression of uPA and Cath-D in endometrial carcinoma group was positively correlated (r = 0.673, P <0.05). Conclusions: Both uPA and Cath-D play a synergistic role in the development, invasion and metastasis of endometrial carcinoma. Cath-D induces the activation of uPA and promotes the invasion and metastasis of cancer cells. Therefore, the combined detection of both may have Help to become an important indicator to judge the development and prognosis of endometrial cancer.