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目的：观察幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒ ｐｙｌｏｒｉ，Ｈｐ）感染对其相关性胃病的细胞增殖与凋亡的影响，进而探讨胃癌的发生机理。方法：研究对象为正常人（ｎｏｒｍａｌ ｓｕｂｊｅｃｔｓ，ＮＳ）及慢性浅表性胃炎（ｃｈｒｏｎｉｃ ｓｕｐｅｒｆｉｃｉａｌｇａｓｔｒｉｔｉｓ， ＣＳＧ）、慢性萎缩性胃炎（ｃｈｒｏｎｉｃ ａｔｒｏｐｈｉｃ ｇａｓｔｒｉｔｉｓ， ＣＡＧ）、慢性萎缩性胃炎伴肠上皮化生 （ｃｈｒｏｎｉｃ ａｔｒｏｐｈｉｃｇａｓｔｒｉｔｉｓ ｗｉｔｈ ｉｎｔｅｓｔｉｎａｌ ｍｅｔａｐｌａｓｉａ，ＣＡＧＩＭ）、不典型增生（ｄｙｓｐｌａｓｉａ， ＤＹＳ）、胃癌（ｇａｓｔｒｉｃ ｃａｎｃｅｒ， ＧＣ） Ｈｐ阳性与阴性患者，共计１１组，每组１０例。细胞凋亡检测采用Ｔｕｎｅｌ方法，细胞增殖检测采用免疫组化ＬＳＡＢ方法测定增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌ ｎｕｃｌｅａｒ ａｎｔｉｇｅｎ，ＰＣＮＡ）。结果： ＣＳＧ Ｈｐ阳性患者细胞凋亡指数（ａｐｏｐｔｏｓｉｓ ｉｎｄｅｘ， ＡＩ）明显高于Ｈｐ阴性患者（ Ｐ＜０．０５），其余４种胃病 Ｈｐ阳性与阴性的患者 ＡＩ比较均无显著性差异（Ｐ＞ ０．０５）； ＣＳＧ、 ＣＡＧ。ＤＹ
Objective: To observe the effect of Helicobacter pylori (Hp) infection on the proliferation and apoptosis of gastric cancer cells in order to explore the mechanism of gastric cancer. Methods: The subjects were normal subjects (NS) and chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), chronic atrophic gastritis with chronic atrophic gastritis with intestinal metaplasia (CAGIM), dysplasia (DYS) and gastric cancer (GC) positive and negative Hp patients, a total of 11 groups, 10 cases in each group. Tunel method was used to detect apoptosis, and proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical LSAB method. Results: The apoptosis index (AI) in CSG Hp positive patients was significantly higher than that in Hp negative patients (P <0.05). There was no significant difference in AI between Hp positive and negative patients in the other 4 kinds of gastric diseases (P> 0.05); CSG, CAG. DY