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利用等电聚焦（ＩＥＦ）电泳和不连续醋酸尿素聚丙烯酰胺凝胶电泳（ＮＡＵ－ＰＡＧＥ）对玉米籽粒贮藏蛋白的等电点（ｐＩ）和在Ｆ１中的遗传表现以及贮藏蛋白在胚和胚乳中的分布进行研究，结果表明：（１）玉米籽粒贮藏蛋白的ｐＩ在３．５－８．４５范围内，可分离的有３９条左右，６０％左右的蛋白质属酸性，ｐＩ分布在３．５０－６．８５范围内，４０％左右属中性偏碱，ｐＩ在６．８５－８．４５范围内。（２）籽粒贮藏蛋白在Ｆ１代呈共显性遗传，没有偏亲现象。所有被测组合在Ｆ１代均有互补带，这是区别自交系与杂交种有效的生化依据。（３）蛋白质在胚中百分含量较高，即浓度较高，胚乳中较低。
The isoelectric point (pI) of maize grain storage protein and its genetic expression in F1 were determined by isoelectric focusing (IEF) electrophoresis and discontinuous urea polyacrylamide gel electrophoresis (NAU-PAGE), and the storage proteins in embryos and endosperm The results showed that: (1) The pI of maize grain storage protein was in the range of 3.5-8.45, and about 39 were separable. About 60% of the proteins were acidic and pI was distributed in 3. In the range of 50-6.85, about 40% is neutral and alkaline, and the pI is in the range of 6.85-8.45. (2) Grain storage protein co-dominant inheritance in F1 generation, there is no partial phenomenon. All tested combinations have complementary bands in F1 generation, which is an effective biochemical basis for distinguishing inbred lines and hybrids. (3) protein in the embryo in a higher percentage, that is, a higher concentration of endosperm lower.