论文部分内容阅读
目的：探讨白血病患者骨髓细胞中载脂蛋白Ｅ（ＡｐｏＥ） 的表达。方法：应用免疫组化ＳＰ法检测正常人骨髓细胞及白血病患者骨髓细胞ＡｐｏＥ的表达，正常人骨髓涂片６ 例，急性淋巴细胞性白血病１３ 例，急性髓性细胞白血病２９ 例。结果：ＡｐｏＥ在所检正常人骨髓涂片中均表达（ 着色程度＋ ～《） ，骨髓细胞表达阳性率为２０％ ±４％ 。急性淋巴细胞白血病原始细胞中不表达ＡｐｏＥ，急性髓性细胞白血病原始细胞不同程度表达ＡｐｏＥ，其中急性粒细胞白血病部分分化型着色＋ ，原始细胞表达阳性率为７０％ ±３２ ％ ，急性早幼粒细胞白血病着色《～》，阳性率８９％ ±４ ％ ，急性粒单细胞白血病着色（＋ ～《），阳性率５０％ ±３％ ，急性单核细胞白血病着色《，阳性率１５ ％ ±４ ％ 。结论：ＡｐｏＥ可作为鉴别髓性和淋巴细胞白血病的一项指标
Objective: To investigate the expression of apolipoprotein E (ApoE) in myeloid cells of leukemia patients. METHODS: Immunohistochemical SP method was used to detect the expression of ApoE in normal bone marrow cells and leukemia patients. Normal bone marrow smears were used in 6 cases, acute lymphoblastic leukemia in 13 cases, and acute myeloid leukemia in 29 cases. Results: ApoE was expressed in normal human bone marrow smears (degree of coloration + ~ ), and the positive rate of bone marrow cells was 20% ± 4%. ApoE is not expressed in blast cells of acute lymphoblastic leukemia. ApoE is expressed in different degrees in acute myelocytic leukemia blast cells. Among them, acute myelogenous leukemia is partially differentiated and the positive rate of primordial cells is 70% +/- 32%. Cellular leukemia coloring “~”, the positive rate of 89% ± 4%, acute unicellular leukemia coloring (+ ~ “), positive rate of 50% ± 3%, acute monocyte leukemia coloring ”, positive rate of 15% ± 4% . Conclusion: ApoE can be used as an indicator to identify myeloid and lymphocytic leukemia