论文部分内容阅读
以小麦成熟胚为外植体,研究了基本培养基、预处理类型、接种方式、植物激素的浓度和不同组合以及分化培养基中是否添加抗生素对愈伤组织诱导和分化的影响,在此基础上建立了一套高效的小麦成熟胚植株再生系统。经过试验,我们选择在MS培养基上接种经无菌水预处理的纵切成熟胚作为起始的试验条件。在含2mg/L 2,4-D的MS培养基上,初级愈伤组织的诱导频率可达80%以上,在继代培养基中添加0.5mg/L 6-BA和0.2mg/L NAA可以显著提高胚性愈伤组织的产生。而在再生培养基中加入适当浓度的头孢霉素可以有效提高胚性愈伤组织再生出小植株的比例。利用该再生系统,我们从5个小麦优良主栽品种的成熟胚再生出了可育的植株,再生频率达15.3%-34.5%。
Using the mature embryo of wheat as explant, the effects of basic medium, pretreatment type, inoculation method, concentration of plant hormone and different combinations of antibiotics on differentiation and differentiation of callus were studied. Based on this, On the establishment of a set of efficient wheat mature embryo plant regeneration system. After testing, we chose to inoculate MS media with sterilized water pre-treatment of mature longitudinal embryos as the initial test conditions. The primary callus induction frequency was more than 80% on MS medium containing 2mg / L 2,4-D, 0.5mg / L 6-BA and 0.2mg / L NAA could be added in the subculture medium Significantly increased embryogenic callus production. However, adding appropriate concentration of cefotaxime to regeneration medium can effectively increase the proportion of embryogenic callus to regenerate plantlets. Using this regeneration system, we regenerated fertile plants from the mature embryos of five elite main cultivars, with regeneration frequencies of 15.3% -34.5%.