论文部分内容阅读
[目的]探讨汉黄芩素对脂多糖(LPS)诱导的BV2细胞激活时所分泌的一氧化氮的产生以及诱导型一氧化氮合酶(iNOS)表达的影响.[方法]用10,100,500,1 000μg/L的LPS刺激小胶质细胞株BV2,并向LPS(100μg/L)中加入10,20,30μmol/L的汉黄芩素后再培养24h,采用Greiss法测定细胞外液所分泌的一氧化氮含量;应用Western-Blot及RT-PCR法分别检测iNOS蛋白和mRNA的表达.[结果]LPS高度激活BV2细胞,并且能使一氧化氮分泌增加,汉黄芩素呈剂量依赖性抑制一氧化氮的含量,同时抑制iNOS蛋白和mRNA的表达.[结论]汉黄芩素可有效抑制LPS激活的BV2细胞iNOS蛋白和mRNA的表达及一氧化氮的产生.
[Objective] To explore the effect of wogonin on the production of nitric oxide and the expression of inducible nitric oxide synthase (iNOS) secreted by lipopolysaccharide (LPS)-induced activation of BV2 cells. [Method] 10, 100, 500, 1 000 μg LPS stimulated the microglial cell line BV2 and added 10, 20, and 30 μmol/L wogonin to LPS (100 μg/L) for 24 h. The Greiss method was used to measure the secretion of oxidized NO in the extracellular fluid. Nitrogen content; The expression of iNOS protein and mRNA was detected by Western-Blot and RT-PCR, respectively. [Results] LPS highly activated BV2 cells and increased the secretion of nitric oxide. Wogonin inhibited nitric oxide in a dose-dependent manner. The content of iNOS protein and mRNA is inhibited. [Conclusion] Wogonin can effectively inhibit the expression of iNOS protein and mRNA and the production of nitric oxide in LPS-activated BV2 cells.