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目的:比较朱砂与氯化汞对大鼠肾功能及肾转运体基因表达的影响差异。方法:将健康雄性SD大鼠随机分为正常组、朱砂组、朱砂安神丸组、氯化汞组,分别灌胃给予等量生理盐水、0.15g·kg-1朱砂、1.4g·kg-1朱砂安神丸、0.02g·kg-1氯化汞,连续给药21d后收集大鼠24h尿液送检,断头取血、肾组织,测定大鼠尿常规、尿素、肌酐、尿酸、白蛋白含量,血肌酐、血尿素氮含量,记录大鼠肾重,计算肾脏指数,RT-PCR法测定肾组织Oat1、Oat3、Aqp1基因的表达。结果:氯化汞组大鼠肾汞蓄积量增高明显,大鼠肾重、肾脏指数、尿素、尿白蛋白含量明显增加,Oat3、Aqp1基因的表达明显降低。朱砂和复方朱砂安神丸组大鼠各项指标均与正常组无明显差异。结论:临床短期使用朱砂及朱砂安神丸未见明显汞蓄积,大鼠肾功能正常,汞转运体基因表达未见明显改变,与氯化汞相比存在显著差异。
Objective: To compare the effects of cinnabar and mercuric chloride on renal function and renal transporter gene expression in rats. Methods: Healthy male Sprague-Dawley rats were randomly divided into normal group, cinnabar group, cinnabar and Anshen Pill group and mercuric chloride group. The rats were given intragastric administration of 0.15g · kg-1 cinnabar, 1.4g · kg-1 Cinnabar and Anshen Pills, 0.02g · kg-1 mercuric chloride. After 21 days of continuous administration, the urine of rats was collected for 24 hours, and the blood and kidney were decapitated. The urine, urine creatinine, uric acid and albumin Serum creatinine and blood urea nitrogen were measured. The kidney weight of rats was recorded and the renal index was calculated. The expression of Oat1, Oat3 and Aqp1 in renal tissues was detected by RT-PCR. Results: The amount of renal Hg accumulation in mercuric chloride group increased significantly, and the kidney weight, kidney index, urea and urinary albumin content increased obviously, while the expression of Oat3 and Aqp1 genes decreased obviously. The indexes of cinnabar and compound cinnabar and Anshen pill group had no significant difference with normal group. Conclusion: There is no significant mercury accumulation in cinnabar and cinnabar Anshen tablets in clinical practice, normal renal function in rats, and no significant change in gene expression of mercury transporter, which is significantly different from that of mercuric chloride.