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目的对福建省一起流行性腮腺炎(腮腺炎)爆发疫情进行病毒分离,了解其基因特征。方法应用非洲绿猴肾细胞分离病毒,逆转录-聚合酶链反应扩增小疏水蛋白(Small Hydrophobic Protein,SH)基因片段,测序并分析其基因特征。结果从爆发点采集的4例标本中,分离到3株腮腺炎病毒,均属于G基因型。此福建株与国内其他省流行的F基因型病毒株核苷酸差异性>9%,与S(上海,Shanghai)79疫苗株SH基因核苷酸差异17.6%,与1999～2005年英国流行的G2基因亚型病毒株核苷酸同源性最高(97.4%～99.8%)。结论此起爆发为G基因型腮腺炎病毒引起,是否为输入性病毒?目前在中国使用的疫苗所产生的抗体能否完全阻断?需进一步加强监测。
Objective To isolate the virus from an epidemic of mumps (Mumps) outbreak in Fujian Province and investigate its genetic characteristics. Methods Virus was isolated from green monkey kidney cells of Africa. Small Hydrophobic Protein (SH) gene fragment was amplified by reverse transcription - polymerase chain reaction (PCR) and sequenced and characterized. Results Four mumps viruses were isolated from the four specimens collected at the outbreak, all of which belonged to G genotype. The Fujian and other provinces of the popular F genotype virus nucleotide diversity> 9%, and S (Shanghai, Shanghai) 79 vaccine strains SH gene nucleotide difference of 17.6%, and 1999-2005 popular in the United Kingdom G2 gene has the highest nucleotide homology (97.4% -99.8%). Conclusions This outbreak is caused by the G genotype mumps virus, is it an imported virus? Can antibodies produced by the vaccines currently used in China be completely blocked? Monitoring needs to be further strengthened.