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目的通过比较正常足月妊娠产妇和同期羊水过少产妇胎盘组织及外周血中人类白细胞抗原-G5(HLA-G5)的不同表达,旨在研究该免疫因子在母婴循环中的意义。方法通过流式细胞学技术,选择20例羊水过少产妇及15例同期正常产妇,检测HLA-G5因子在胎盘和外周血组织中的数值的表达,分析其在羊水过少发生中的意义。结果实验组(羊水过少产妇)与对照组(正常产妇)胎盘组织中CD4+HLA-G5+T淋巴细胞分别为0.68±0.32,1.91±1.14(P<0.05),而CD8+HLA-G5+T淋巴细胞所占比例分别为67.52±15.99,67.52±15.99(P<0.05).实验组与对照组外周血中分别对照分析结果均为(P>0.05)。结论 HLA-G5+在胎盘中的低表达可能与羊水过少的发生有关,对母胎液体交换有意义。
Objective To compare the different expression of human leukocyte antigen-G5 (HLA-G5) in normal pregnant women and oligohydramnios mothers placenta and peripheral blood in order to study the significance of this immune factor in maternal-fetal cycle. Methods Flow cytometry was used to detect the expression of HLA-G5 in placenta and peripheral blood of 20 cases of oligohydramnios and 15 cases of normal pregnant women. The significance of HLA-G5 expression in oligohydramnios was analyzed. Results The percentage of CD4 + HLA-G5 + T lymphocytes in placenta of experimental group (n = 30) and control group (normal pregnant women) were 0.68 ± 0.32 and 1.91 ± 1.14 (P <0.05) T lymphocytes were 67.52 ± 15.99,67.52 ± 15.99 respectively (P <0.05), and the results of control analysis in the peripheral blood of the experimental group and the control group were respectively (P> 0.05). Conclusions The low expression of HLA-G5 + in the placenta may be related to the occurrence of oligohydramnios, which is of significance for the exchange of fetal fluid.