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采用免疫组织化学方法检测了30例肝细胞癌（HCC）组织P53基因蛋白产物及癌组织中增殖细胞核抗原（PCNA）的表达。肝癌组织P53基因蛋白的异常表达率为46．7％；在分化程度为Ⅰ、Ⅱ、Ⅲ级癌组织的表达率分别为14．3％、40．0％、87．5％（P＜0．05）。PCNA阳性细胞的分布在HCC中呈4种类型：散在型、边缘型、镶嵌型及弥漫型；其标记指数（LI）与癌细胞的分级相关．P53基因蛋白表达异常的癌组织中PC．NALI（53．8±24．3）明显高于无异常者（33．1±15．7）（P＜0．05）．上述结果揭示P53基因的突变在部分HCC的发生发展过程中起一定的作用，PCNA的表达与HCC的分化程度相关，伴P53基因蛋白的HCC癌细胞增生活跃，恶性程度高．
Immunohistochemical method was used to detect the expression of P53 protein in hepatocellular carcinoma (HCC) tissue and the expression of proliferating cell nuclear antigen (PCNA) in cancer tissues. The abnormal expression rate of P53 gene protein in hepatocellular carcinoma was 46.7%, and the expression rates of I, II, and III cancer tissues were 14.3%, 40.0%, and 87.5%, respectively (P<0). .05). There are four types of PCNA-positive cells in HCC: scattered type, borderline type, mosaic type and diffuse type. The labeling index (LI) is related to the grade of cancer cells. P53 gene protein expression is abnormal in the cancer tissue in PC. NALI (53.8±24.3) was significantly higher than that without abnormality (33.1±15.7) (P<0.05). The above results revealed that the mutation of P53 plays a role in the development of some HCCs. The expression of PCNA is related to the degree of differentiation of HCC, and the HCC cancer cells with P53 gene protein are hyperactive and highly malignant.