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目的对深圳地区分离到的新甲型H1N1(2009)流感病毒的M基因分子特性进行详细分析,并从分子水平上了解其对金刚烷胺类药物的耐药性。方法选取深圳地区分离培养到的55株新甲型H1N1(2009)流感病毒,对其M片段进行全长测序,采用DNAstar与MEGA3.1生物软件对M基因序列进行比对并用NJ法构建系统进化树。结果通过对M基因的氨基酸序列进行进化分析,发现深圳地区分离培养到的新甲型H1N1(2009)流感病毒更接近欧洲猪流感病毒,与季节性H1N1流感病毒差异很大。经过序列分析发现,深圳株的M基因与A/California/07/2009高度同源,显示该病毒M基因较保守。此外,还发现所有深圳株的M2蛋白均出现S31N突变,显示对金刚烷胺类药物可能耐药。结论深圳流行的新甲型H1N1(2009)流感病毒的M基因可能源于欧洲猪流感病毒,且均对金刚烷胺类药物有耐药倾向。应进一步加强对新甲型H1N1(2009)流感病毒的分子特征研究,并积极开展流感病毒耐药性监测。
Objective To analyze the M gene molecular characteristics of the influenza A (H1N1) virus isolated in Shenzhen and to understand its resistance to amantadine at the molecular level. Methods Fifty-five new H1N1 (2009) influenza viruses isolated and cultured in Shenzhen were selected and their M fragments were sequenced. The sequence of M gene was aligned by DNAstar and MEGA3.1 software and the phylogenetic tree was constructed by NJ tree. Results By analyzing the amino acid sequence of M gene, it was found that the influenza A (H1N1) virus isolated and cultivated in Shenzhen was closer to the European swine flu virus, which was different from the seasonal H1N1 influenza virus. After sequence analysis, the M gene of Shenzhen strain was highly homologous to A / California / 07/2009, indicating that the M gene of the strain is more conservative. In addition, all S31N mutations were found in M2 protein of Shenzhen strain, indicating that they may be resistant to amantadine drugs. Conclusion The M gene of new influenza A H1N1 (2009) influenza virus circulating in Shenzhen may be derived from the European swine flu virus, and all of them are resistant to amantadine drugs. Should further strengthen the new H1N1 (2009) influenza virus molecular characteristics of research, and actively carry out influenza virus drug resistance monitoring.