【摘 要】
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OBJECTIVE:To find out the combination of the extracts from Paeonia lactiflora Pallas (PL),Rehmannia Glutinosa var.Purpurea Makino (RG),Perilla Frutescens var.Ac
【机 构】
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School of Korean Medicine and Korean Medical Research Center for Healthy Aging,Pusan National Univer
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OBJECTIVE:To find out the combination of the extracts from Paeonia lactiflora Pallas (PL),Rehmannia Glutinosa var.Purpurea Makino (RG),Perilla Frutescens var.Acuta Kudo (PF) to increase endometrial receptivity.METHODS:Herbal medicines were extracted with boiling water and polysaccharides were removed.We examined the effect of PL,RG,and PF (PRP),a most effective herbal formula deduced from constitutive ingredient herbs of Antai Yin which is composed of PRP,on the leukemia inhibitory factor (LIF) expression and endometrial receptivity.RESULTS:The combination of the extracts from PRP induced the LIF expression in Ishikawa cells and increased the adhesion between Ishikawa and JAr cells.In addition,PRP-induced attachment of JAr cells onto Ishikawa cells and expression of adhesion molecules,ITGAV,ITGB5,CD44s,and L-selectin,are significantly reduced by knock-down of LIF expression.CONCLUSION:Induced by the combination of the PRP extracts,the adhesion between trophoblast and endometrial cells are mediated by expression of LIF and adhesion molecules.Thus,we suggest the combination of the PRP extracts may be a novel therapy for enhancing embryo implantation rate.
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