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目的:观察复方黄芪当归汤对MS大鼠肾损害的干预作用,论证其在诊治MS方面的药用价值。方法:制备大鼠代谢综合征模型,观察正常对照组、模型组、预防组、中药治疗组以及西药对照组,各组用药前后肾脏组织形态学病理改变,并观察对肾小管组织中MMPs及TIMPs的水平表达的影响。结果:(1)干预4周后,除预防组外各组大鼠的体质量均较正常对照组明显增加,差异显著(P<0.05);(2)与正常对照组相比,各组大鼠MMP-2、TIMP-2、MMP-9、TIMP-1的mRNA相对表达量均显著增加,MMP-2/TIMP-2,MMP-9/TIMP-1比值均低于正常对照组(P<0.05);(3)各组大鼠MMP-2、TIMP-2、MMP-9、TIMP-1的mRNA相对表达量均低于模型组,MMP-9/TIMP-1、MMP-2/TIMP-2比值均高于模型组,具有统计学差异(P<0.05)。结论:(1)复方黄芪当归汤预防和治疗组能减少MS模型大鼠肾组织MMP-2、TIMP-2、MMP-9、TIMP-1的mRNA表达,并升高MMP-2/TIMP-2、MMP-9/TIMP-1比值,改善MMPs/TIMPs平衡。(2)复方黄芪当归汤预防和治疗组能减轻MS模型大鼠的肾脏损伤。
Objective: To observe the intervention effect of compound astragalus and angelica decoction on renal damage in MS rats, and to demonstrate its medicinal value in diagnosis and treatment of MS. Methods: The rat model of metabolic syndrome was prepared and the normal control group, model group, prophylaxis group, traditional Chinese medicine treatment group and western medicine control group were observed. The morphological changes of renal tissues were observed before and after treatment. The expressions of MMPs and TIMPs The impact of the level of expression. Results: (1) After 4 weeks of intervention, the body weight of rats in each group was significantly higher than that of the control group except for the prevention group (P <0.05); (2) Compared with the normal control group, The relative mRNA expression of MMP-2, TIMP-2, MMP-9 and TIMP-1 were significantly increased, while the ratios of MMP-2 / TIMP-2 and MMP-9 / TIMP-1 were significantly lower than those of the normal control group 0.05). (3) The mRNA expressions of MMP-2, TIMP-2, MMP-9 and TIMP-1 in rats in each group were lower than those in model group 2 ratio were higher than the model group, with statistical significance (P <0.05). Conclusion: (1) Compound Astragalus and Angelicum decoction can reduce the mRNA expression of MMP-2, TIMP-2, MMP-9 and TIMP-1 and increase the expressions of MMP-2 / TIMP-2 in MS model rats , MMP-9 / TIMP-1 ratio, improve the balance of MMPs / TIMPs. (2) Compound Astragalus and Angelicae Soup prevent and treat the kidney damage of MS model rats.