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目的建立和优化脑膜炎奈瑟菌(Neisseria Meningitidis,Nm)对人喉癌上皮细胞(Human Larynx Carcinoma Epithelial Cell,HEp-2)的黏附实验方法,检测C群序列型(SequenceType,ST)4821序列群和不可分群(Nongroupable,NG)Nm对HEp-2黏附能力的差异性。方法以C群Nm疫苗株C11作为靶菌,选用HEp-2,选择不同的黏附时间和不同的感染复数(Multiplicity of Infection,MOI)确定Nm与HEp-2黏附作用的最适条件,对中国分离的26株C群ST-4821序列群和8株NG Nm菌株进行HEp-2黏附能力研究。结果 Nm与HEp-2黏附研究最适作用时间为4h,最适MOI为100～1000;各血清群参考菌株的黏附菌落数在1.46×105～1.63×106菌落形成单位/毫升(Colony Form ingUnit,CFU/ml);26株ST-4821序列群的C群Nm对HEp-2的黏附菌落数在1.33×105～9.53×106CFU/ml;8株NGNm的黏附菌落数在3.04×105～8.52×106CFU/ml。结论不同血清群Nm对HEp-2的黏附能力存在差异;同是ST-4821序列群的C群Nm对HEp-2的黏附能力存在差异;同是NG Nm对HEp-2的黏附能力存在差异。
Objective To establish and optimize the adhesion assay of Neisseria meningitidis (Nm) to Human Larynx Carcinoma Epithelial Cell (HEp-2) and to detect the sequence of 4821 Sequence Group (ST) And Nongroupable (NG) Nm on the adhesion of HEp-2. Methods Cn group Nm vaccine strain C11 was used as target bacteria. HEp-2 was selected and different adhesion times and different multiplicity of infection (MOI) were selected to determine the optimum conditions for the adhesion of Nm to HEp-2. Of 26 C group ST-4821 sequence group and 8 NG Nm strains for HEp-2 adhesion. Results The optimal time for adhesion between Nm and HEp-2 was 4h and the optimum MOI was 100-1000. The number of colonies adhered to reference strains of each serogroup was between 1.46 × 105 and 1.63 × 106 Colony Forming Units / ml (Colony Form ing Unit, CFU / ml). The number of colonies adhered to HEp-2 by C group Nm of 26 strains of ST-4821 was 1.33 × 105-9.53 × 106CFU / ml. The number of colonies adhered to 8 strains of NGNm was 3.04 × 105-8.52 × 106CFU / ml. Conclusions The adhesion ability of Np to HEp-2 is different among different serogroups. The adhesion of HEp-2 to C-group Nm of ST-4821 is also different. The same is the difference of the adhesion ability of NG Nm to HEp-2.