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目的研究局部脑缺血再灌注后N-甲基-D-天冬氨酸(NMDA)受体I型亚单位(NR1)mRNA表达与细胞凋亡的关系及银杏叶制剂(Egb)对其影响。方法140只SD大鼠被随机分成假手术组、缺血再灌组,即MCAO组和Egb治疗组。每组大鼠再随机分成4组:分别在再灌注后6,24,48,96 h处死。进行TUNEL染色检测缺血区细胞凋亡情况,并用原位杂交方法检测NR1 mRNA。结果MCAO组大鼠大脑皮质梗死灶的凋亡细胞和NR1mRNA表达与假手术组比较明显增强(P<0.01),而Egb治疗组这两项指标的表达明显低于MCAO组(P<0.01)。结论再灌注后脑组织中NR1mRNA表达增强,与细胞凋亡密切相关。银杏叶制剂可以明显抑制NR1mRNA的表达,减轻细胞凋亡。
Objective To study the relationship between the expression of NMDA receptor type I subunit (NR1) mRNA and cell apoptosis after focal cerebral ischemia-reperfusion in rats and the effect of ginkgo biloba leaf preparation (Egb) on it. . Methods One hundred and forty Sprague-Dawley rats were randomly divided into sham operation group and ischemia reperfusion group, MCAO group and Egb treatment group. Each group of rats was randomly divided into 4 groups: they were sacrificed at 6, 24, 48, and 96 h after reperfusion. TUNEL staining was used to detect apoptosis in the ischemic area, and NR1 mRNA was detected by in situ hybridization. Results The expression of apoptotic cells and NR1 mRNA in the infarcted cerebral cortex of MCAO rats was significantly increased compared with the sham group (P<0.01). The expression of these two indexes in the Egb treatment group was significantly lower than that in the MCAO group (P<0.01). Conclusion The expression of NR1 mRNA in brain tissue is increased after reperfusion, which is closely related to apoptosis. Ginkgo biloba extract can significantly inhibit the expression of NR1 mRNA and reduce apoptosis.