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本文介绍一个不连续缓冲系的聚丙烯酰胺凝胶圆盘电泳和一个新的活性染色程序。用牛犊小肠和人胎盘硷性磷酸酶作内标和外标,分离鉴定人血清碱性磷酸酶同工酶,所得电泳图谱与萘基磷酸固兰 BB 盐染色改进法比较,清晰度、灵敏性、和重现性均较好,可以扫描定量。分析120例以上体检和肝病血清,各同工酶(肝、骨、肠和其它高分子量碱性磷酸酶四带)电泳迁移率与文献参考比值完全一致。
This article describes a discontinuous buffer polyacrylamide gel disk electrophoresis and a new reactive staining program. Using calf intestine and human placental alkaline phosphatase as internal standard and external standard, human serum alkaline phosphatase isoenzyme was isolated and identified. The obtained electrophoretic pattern was compared with the improved method of naphthalene phosphoric acid solid blue BB salt stain, the definition, sensitivity, And reproducibility are better, you can scan the quantitative. Analysis of more than 120 cases of physical examination and liver disease serum, isoenzyme (liver, bone, intestine and other high-molecular weight alkaline phosphatase four bands) electrophoretic mobility and the reference ratio is consistent with the literature.