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近年来,有报道说静脉采血可使某些患者的精神紧张,使血中某些激素水平突然升高,而使测定结果不准确。另外,血中激素的测定值为血循环中的总浓度,不能反映有生物学活性的游离激素水平。唾液中不含性激素结合蛋白,故唾液中的性甾体激素的浓度能较好地反映有生物学活性的游离激素水平。采用以氚为标记物的RIA测定性甾体激素水平,其灵敏度较高,但操作程序繁杂。而用以碘为标记的RIA,进行测定,操作简单,但灵敏度不够。经反复试验,我们改进了以碘标记的RIA操作方法,其标记品和抗
In recent years, it has been reported that venous blood sampling can cause mental stress in some patients, causing some hormone levels in the blood to rise abruptly, rendering the measurement inaccurate. In addition, the measured value of blood hormone is the total concentration in the blood circulation and does not reflect the level of free-hormone with biological activity. Saliva contains no sex hormone binding protein, so salivary sex steroid hormone concentration can better reflect the biological activity of free hormone levels. The use of tritiated RIA determinative steroid hormone levels, the higher the sensitivity, but the operating procedures complicated. RIA labeled with iodine was measured, the operation is simple, but the sensitivity is not enough. After trial and error, we have improved the RIA method of iodine labeling, its labeling and resistance