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目的:探讨超声微泡介导转染FKBP12.6基因后,对小鼠H9c2(2-1)心肌细胞中Ca2+浓度的影响。方法:将pcDNA3.1-FKBP12.6质粒与白蛋白包裹微泡造影剂混合,经超声转染H9c2(2-1)细胞后,通过倒置显微镜观察心肌细胞生长状况的变化;激光共聚焦显微镜检测细胞内Ca2+浓度的变化;免疫组织化学方法检测FKBP12.6蛋白的表达。结果:超声触发微泡破裂转染的FKBP12.6基因可在心肌细胞高效表达,细胞生长良好。高表达FKBP12.6的心肌细胞中,总的钙离子浓度增加。结论:超声微泡介导FKBP12.6基因转染心肌细胞,可以明显增加心肌细胞中的Ca2+浓度,心肌细胞的收缩能力增强。
Objective: To investigate the effect of ultrasound microbubble mediated transfection of FKBP12.6 gene on Ca2 + concentration in mouse H9c2 (2-1) cardiomyocytes. Methods: The pcDNA3.1-FKBP12.6 plasmids were mixed with albumin-coated microbubble contrast agent and the H9c2 (2-1) cells were transfected by ultrasound. The changes of the growth of cardiomyocytes were observed by inverted microscope. The confocal laser scanning microscopy Intracellular Ca2 + concentration changes; immunohistochemistry method to detect FKBP12.6 protein expression. Results: Ultrasound-triggered microbubble rupture transfected FKBP12.6 gene can be highly expressed in cardiomyocytes, cells grow well. In cardiomyocytes that are highly expressed FKBP12.6, the total calcium concentration is increased. Conclusion: Ultrasound microbubble-mediated FKBP12.6 gene transfection of myocardial cells, can significantly increase the concentration of Ca2 + in myocardial cells, myocardial contractility increased.