欧李愈伤组织诱导及分化研究

• 作 者: 胡延生 姜继发 建德锋
• 机 构: 吉林农业科技学院,吉林省镇赉县园林管理中心,
• 出 处: 吉林农业科学
• 发表日期: 2015年06期
• 关键词: 愈伤组织诱导 欧李 培养基 愈伤组织 分化研究 分化率 外植体 不定芽 分化情况 分化培养基

为了快速大量繁育欧李苗木,尝试采用离体繁殖方法进行繁育。该试验以欧李茎尖为外植体,研究了在诱导阶段不同培养基配方对其愈伤组织诱导及分化的影响。结果表明愈伤组织诱导培养基以MS+KT0.6 mg/L配方较好,诱导率可达62%;愈伤组织分化选用MS+KT 0.5 mg/L+IBA 0.2 mg/L或MS+KT 1.0 mg/L+IBA0.2 mg/L培养基均较好,分化率可达80%,且能较早完成分化,并且分化不定芽数量较多。

In order to massively breed European plum seedlings, try breeding using in vitro breeding methods. In this experiment, the stems of P. przewalskii were used as explants to study the effects of different media formulations on the induction and differentiation of callus during the induction phase. The results showed that the induction rate of callus induction medium with MS + KT0.6 mg / L was up to 62%, and the callus differentiation was MS + KT 0.5 mg / L + IBA 0.2 mg / L or MS + KT 1.0 mg / L IBA0.2 mg / L medium were better, the rate of differentiation up to 80%, and can complete the differentiation earlier, and the number of adventitious buds are more.