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为了解在同地区内、条件相同情况下献血员与同类人群间抗-HCV感染的特征,我们在1992年进行了献血员抗-HCV的对照调查。调查对象:NBsAg阴性、ALT正常的3259名农村献血员为调查对象。对照组全部来自农村,为与献血员年龄、居住条件相同、无献血史者,经整群抽样共1268名。方法:无菌静脉采血3ml,分离血清于-20℃保存待检。采用ELISA法检测抗-HCV。试剂为沈阳惠民医药试剂研究所生产,批号为920712、920812均在有效期检测,在450nm波长下用酶标仪测其OD值,P/N值≥
To understand the characteristics of anti-HCV infection among blood donors and their cohorts under the same conditions in the same area, we conducted a controlled survey of anti-HCV blood donors in 1992. Survey: NBsAg negative, normal ALT 3259 rural blood donors as the survey. The control group all came from rural areas. For the same age and living conditions as blood donors, there were 1268 sampled persons in a cluster. Methods: sterile venous blood 3ml, the sera were stored at -20 ℃ to be tested. Anti-HCV was detected by ELISA. Reagents for the Shenyang Institute of Pharmaceutical Reagent manufacturers, batch number 920,712,920,812 are tested in the validity of 450nm wavelength with the microplate reader measured OD value, P / N value ≥