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观察NF-κB在抗人DR5抗体mDRA-6诱导白血病细胞凋亡中的激活,探讨NF-κB在抗人DR5抗体诱导白血病细胞凋亡中的作用。提取细胞蛋白,间接ELISA法检测NF-κB的激活;预先应用NF-κB抑制剂孵育细胞1h,MTT及流式细胞术检测mDRA-6对白血病Jurkat和U937细胞系的凋亡率。结果:mDRA-6作用Jurkat和U937细胞15min,激活形式NF-κB(NF-κBP65)明显增高;10mg/L的mDRA-6作用Jurkat和U937细胞4h,流式细胞术检测细胞凋亡率分别为56.63%和34.14%,而预先用15μmol/L的NF-κB抑制剂处理细胞1h,mDRA-6所致的Jurkat和U937细胞凋亡率分别增加至75.51%和58.01%。mDRA-6诱导白血病Jurkat和U937细胞凋亡过程中激活NF-κB,激活的NF-κB抑制mDRA-6的肿瘤细胞凋亡诱导作用。
To observe the activation of NF-κB in anti-human DR5 antibody mDRA-6-induced leukemia cell apoptosis, and to explore the role of NF-κB in anti-human DR5 antibody-induced leukemia cell apoptosis. The cell proteins were extracted and the activation of NF-κB was detected by indirect ELISA. The cells were pre-incubated with NF-κB inhibitor for 1 h. The apoptosis rates of mDRA-6 on Jurkat and U937 cell lines were determined by MTT and flow cytometry. Results: The mDRA-6 treated Jurkat and U937 cells for 15min, the activated form of NF-κB (NF-κBP65) was significantly increased; 10mg / L mDRA-6 Jurkat and U937 cells for 4h, flow cytometry were apoptotic rates were 56.63% and 34.14%, respectively. However, the apoptotic rates of Jurkat and U937 cells induced by mDRA-6 were increased to 75.51% and 58.01% respectively when treated with 15μmol / L NF-κB inhibitor for 1h. Activation of NF-κB in mDRA-6-induced leukemia Jurkat and U937 cells inhibits the induction of apoptosis in mDRA-6 cells by activated NF-κB.