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目的:探讨父源性人类白细胞分化抗原HLA-B基因与子痫前期的相关性。方法:利用流式反向特异序列寡核苷酸探针(PCR-SSO)基因分型技术,对中国地区汉族人群中37对子痫前期患者夫妇及38对正常孕妇夫妇的外周血DNA,进行HLA-B等位基因分型;分别比较两组母亲之间和两组配偶之间等位基因及基因型的频率分布,通过妇/夫基因型配伍,比较两组间基因型配伍频率分布的差异。结果:①子痫前期患者和正常晚孕者中检出34个HLA-B等位基因;配偶中检出28个HLA-B等位基因;两组孕妇及其配偶间HLA-B基因频率均无统计学差异;②子痫前期组妇携带HLA-B13夫不携带HLA-B13基因的频率显著高于正常晚孕组(P<0.05);③子痫前期组夫妇均不携带HLA-B13基因的频率显著低于正常晚孕组(P<0.05)。结论:夫妇间的某些特定HLA-B基因配伍模式可能与子痫前期的发病相关。
Objective: To investigate the relationship between HLA-B gene of paternal human leukocyte differentiation antigen and preeclampsia. METHODS: Peripheral blood DNA from 37 pairs of preeclampsia couples and 38 pairs of normal pregnant women in Chinese Han population were genotyped by PCR-SSO genotyping HLA-B allelic genotypes; frequency distribution of alleles and genotypes between two mothers and two groups of spouses were compared respectively, and the frequency distribution of genotype compatibility between the two groups was compared difference. Results: (1) 34 HLA-B alleles were detected in preeclampsia and normal pregnant women, while 28 HLA-B alleles were detected in the spouses. The frequencies of HLA-B alleles in both groups of pregnant women and their spouses There was no significant difference between the two groups. ②The frequency of HLA-B13 gene carrying HLA-B13 in preeclampsia group was significantly higher than that in normal pregnancy group (P <0.05); ③ The pre-eclampsia group did not carry HLA-B13 gene The frequency was significantly lower than the normal pregnancy group (P <0.05). Conclusion: Some patterns of HLA-B compatibility between couples may be related to the pathogenesis of preeclampsia.