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目的研究卵巢腺癌组织中肿瘤相关巨噬细胞(TAM)、肥大细胞(MC)和微血管(MV)计数,并探讨其临床病理意义及其相互关系。方法43例卵巢腺癌手术切除标本常规制作石蜡包埋切片,TAM,MC和MV染色方法均为EnVisionTM免疫组化法。结果43例卵巢腺癌TAM、MC和MV计数均值分别为14.8±5.2个/HP、6.8±2.4个/HP和58.8±11.4个/HP;组织学分级G1、临床分期Ⅰ+Ⅱ、淋巴结未转移及未侵犯周围组织器官的病例TAM、MC和MV计数均值明显低于组织学分级G3、临床分期Ⅲ+Ⅳ、淋巴结转移及侵犯周围组织器官病例(P<0.01);TAM计数与MC(r=0.42,P<0.01)和MV(r=0.54,P<0.01)计数呈密切正相关,MC计数与MV计数呈密切正相关(r=0.48,P<0.01)。结论TAM、MC和MV计数可能均为反映卵巢腺癌进展、生物学行为及预后的重要标志物,TAM和MC均具有强烈促肿瘤血管生成作用。
Objective To study the counts of tumor-associated macrophages (TAMs), mast cells (MCs) and microvessels (MVs) in ovarian adenocarcinoma and to explore the clinicopathological significance and their relationship. Methods Forty-three surgical specimens of ovarian adenocarcinoma were paraffin-embedded sections. TAM, MC and MV staining were performed by EnVisionTM immunohistochemistry. Results The mean counts of TAM, MC and MV in 43 cases of ovarian adenocarcinoma were 14.8 ± 5.2 / HP, 6.8 ± 2.4 / HP and 58.8 ± 11.4 / HP, respectively. The histological grade G1, clinical stage Ⅰ + Ⅱ, lymph node metastasis (P <0.01). The mean of TAM, MC and MV counts were significantly lower than those of histological grade G3, clinical stage Ⅲ + Ⅳ, lymph node metastasis and surrounding tissues and organs (P <0.01) 0.42, P <0.01) and MV (r = 0.54, P <0.01). There was a positive correlation between MC count and MV count (r = 0.48, P <0.01). Conclusions The counts of TAM, MC and MV may both be important markers of ovarian adenocarcinoma progression, biological behavior and prognosis. Both TAM and MC have a strong effect of promoting tumor angiogenesis.