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目的观察戊地昔布与吡柔比星联合应用对人肺癌A549细胞的抑制效应、细胞周期和凋亡的影响。方法 用四甲基偶氮唑蓝法检测戊地昔布与吡柔比星对人肺癌A549细胞的抑制率,并用中效原理判断二者之间相互作用;用流式细胞术检测肿瘤细胞周期分布和细胞凋亡率;用蛋白质印迹法检测Bcl-2、Bax和caspase-3等凋亡蛋白表达。结果 戊地昔布与吡柔比星联合用药对人肺癌A549细胞的抑制率比二者单独用药时明显增加,合用指数值小于1,细胞凋亡率也明显提高。联合用药后,Bcl-2表达进一步减少、Bax和caspase-3表达进一步增多。结论戊地昔布和吡柔比星联合用药后可通过增加细胞凋亡率而明显增强对A549细胞生长的抑制作用。
Objective To observe the effects of combination of amoxicillin and pirarubicin on the inhibitory effect, cell cycle and apoptosis of human lung cancer A549 cells. Methods MTT assay was used to detect the inhibition rate of amoxicillin and pirarubicin on human lung cancer cell line A549. The interaction between them was evaluated by the principle of neutral effect. The cell cycle of tumor cells was determined by flow cytometry Distribution and apoptosis rate; Western blotting was used to detect the expression of Bcl-2, Bax and caspase-3. Results The combination of amoxicillin and pirarubicin significantly reduced the inhibitory rate of human lung adenocarcinoma A549 cells compared with the control group. When the combination index was less than 1, the apoptosis rate of A549 cells was also significantly increased. After combined treatment, the expression of Bcl-2 further decreased and the expression of Bax and caspase-3 increased further. Conclusion The combination of amoxicillin and pirarubicin can significantly enhance the apoptosis of A549 cells by increasing the apoptosis rate.