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应用聚合酶链反应（ＰＣＲ）结合ＨｐａⅡ限制性内切酶消化法（ＨｐａⅡ－ＰＣＲ）检测５８例急性白血病（ＡＬ）患儿降钙素（ＣＴ）基因的甲基化状态。病例组待检细胞ＤＮＡ经ＨｐａⅡ消化后，再用两对ＣＴ基因特异性引物作ＰＣＲ扩增，分别产生长度为５６６ｂｐ和１．４ｋｂ特异片段。急性淋巴细胞白血病阳性率７１．４％（２５／３５），急性非淋巴细胞白血病７８．２％（１８／２３）。敏感性达１０－３。证明ＣＴ基因５′高度甲基化是白血病细胞克隆的特异标志。本课题受卫生部、四川省人民医院出国人员基金资助
The methylation status of calcitonin (CT) gene in 58 children with acute leukemia (AL) was detected by polymerase chain reaction (PCR) and Hpa Ⅱ restriction endonuclease digestion (HpaⅡ-PCR). In the case group, the DNA to be tested was digested with HpaII, and then amplified with two pairs of CT gene-specific primers to generate specific fragments of length of 566 bp and 1.4 kb, respectively. The positive rate of acute lymphoblastic leukemia was 71.4% (25/35) and acute non-lymphocytic leukemia 78.2% (18/23). Sensitivity of 10-3. It is proved that the 5 ’hypermethylation of CT gene is a specific marker of leukemic cell cloning. The subject by the Ministry of Health, Sichuan Provincial People’s Hospital funded personnel abroad