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目的 揭示连环蛋白与肝癌的发生发展的关系。方法 应用RT -PCT和免疫组织化学观察细胞黏附与信号转导分子 β 和 p12 0 -连环蛋白在正常肝组织、肝癌旁结节性肝硬化组织和肝癌组织中 p12 0 ctnmRNA的蛋白质表达情况及其与β 连环蛋白的关系。 结果 所有肝组织中均表达p12 0 ctnmRNA同工蛋白 1A和 3A。 2例正常肝组织中 β 和p12 0 连环蛋白分子表现为胞膜表达而胞质无表达 ;17例癌旁结节性肝硬化组织表现为胞膜和胞质均有表达 ,以胞质为主 ,且胞膜表达有所增强。 17例HCC组织中表现为胞膜表达明显减弱或消失 ,而胞质表达则增强。多数细胞胞膜表达呈现不连续性。结论 肝组织中可检测到p12 0 ctn同工蛋白 1A和 3AmRNA。此外 ,正常肝组织中 β 和 p12 0 连环蛋白分布在胞膜 ,对维持正常的细胞黏附和信号转导起重要作用 ;结节性肝硬化肝组织中此 2种分子出现了分布变化 ,而肝癌细胞中此 2种分子发生了明显的转位现象 ,提示肝癌细胞在一定程度上改变了细胞黏附分子的正常功能和其所介导的信号转导作用
Objective To reveal the relationship between the development of catenin and liver cancer. Methods RT-PCT and immunohistochemistry were used to observe the protein expression of p12 0 ctn mRNA in the normal liver tissue, hepatic nodular liver cirrhosis tissue and hepatocellular carcinoma, and the cell adhesion and signal transduction molecules β and p12 0-catenin. Relationship with beta catenin. Results The p12 0 ctn mRNA isoforms 1A and 3A were expressed in all liver tissues. In 2 cases of normal liver tissue, β and p12 0 catenin molecules showed membrane expression but no cytoplasmic expression; 17 cases of adjacent nodular cirrhosis showed expression of both cell membrane and cytoplasm, mainly cytoplasmic. , And the membrane expression has been enhanced. In 17 cases of HCC, the expression of membranes was significantly reduced or disappeared, while the cytoplasmic expression was enhanced. Most cell membrane expression shows discontinuity. Conclusion The p12 0 ctn isoform proteins 1A and 3A mRNA can be detected in liver tissue. In addition, beta- and p12 0 catenins in normal liver tissue are distributed in the membrane, and play an important role in maintaining normal cell adhesion and signal transduction; these two kinds of molecules in the liver tissue of nodular cirrhosis have a distribution change, while liver cancer The obvious translocation of these two kinds of molecules in the cells suggests that hepatoma cells change the normal function of cell adhesion molecules and the signal transduction mediated by them.