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探讨聚合酶链反应（ＰＣＲ）技术检测临床标本中军团病杆菌（ＬＤＢ）对军团菌病的诊断价值。用ＰＣＲ技术对３１例ＬＤＢ培养和（或）血清学阳性、临床诊断为军团菌病患者急性期的临床标本中，军团菌巨噬细胞感染增效基因（ｍｉｐｇｅｎｅ）的６３０ｂｐ片段进行ＤＮＡ扩增，并与军团菌培养和血清学诊断（间接荧光抗体检查法）比较。结果：ＰＣＲ技术诊断军团菌病的阳性率为９０．３％（２８／３１），比培养的阳性率（６２．６％，１７／２７）高（χ２＝８．２３，Ｐ＜０．００５），与血清学检测阳性率（８６．２％，２５／２９）之间无显著性差异（χ２＝０．８９，Ｐ＞０．２５）。在ＬＤＢ培养阴性、血清学阴性的４０例肺部感染患者中３例ＰＣＲ法检测结果阳性。结论：ＰＣＲ技术检测临床标本中ＬＤＢ是军团菌病诊断的有效而实用的方法
To investigate the diagnostic value of Polymerase Chain Reaction (PCR) in detection of Legionnaires’ disease in clinical specimens. PCR amplification of 630bp fragments of mipgene of Legionella pneumophila infected by Legionella pneumophila in 31 cases of LDB culture and / or serology positive and clinically diagnosed as acute stage of Legionnaires’ disease, And compared with Legionella cultures and serological diagnosis (indirect fluorescent antibody test). Results: The positive rate of PCR diagnosis of Legionnaires’ disease was 90.3% (28/31), higher than the positive rate of culture (62.6%, 17/27) (χ2 = 8.23, P <0.005 ), And no significant difference from serological test (86.2%, 25/29) (χ2 = 0.89, P> 0.25). Three of the 40 patients with LDB-negative and seronegative lung infections had a positive PCR result. Conclusion: LDB detection in clinical specimens by PCR is an effective and practical method for the diagnosis of Legionnaires’ disease