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1987～1988年在河南新乡用相同接菌量种植感病品种,对棉花抗枯萎病品种86—1连作10年以上田块土样进行盆栽和田间小区试验。结果表明.枯萎病平均病指分别为29.4和4.4,与对照果园土比较抑菌效果分别为39.9％和85.8％。上述田块中86—1棉株根围5cm内土样,盆栽枯萎病平均病指19.5,抑菌效果60.5％。连作10年以上枯萎病圃土样,病指分别为27.2和6.8,抑菌效果为43.9％和76.2％。1990～1991年在辽宁省经作所试验结果,连作10年以上枯萎病圃田间抑菌效果56.2％,连作5年病圃抑菌效果17.6％。上述结果证明,枯萎病圃衰退的主要原因是棉花枯萎病菌抑菌土的产生和存在。土样内枯萎菌菌量测定结果表明,3个抑菌土土样内菌量比果园导菌土减少48.6％～59.2％。抑菌土经高压灭菌后抑菌效果完全消失,初步证明抑菌因子可能以生物因子为主。
From 1987 to 1988, the susceptible cultivars were planted in Xinxiang, Henan with the same inoculum size, and potted and field plot test was conducted on the plots of above plots with the resistance to cotton wilt disease 86-1 for more than 10 years. The results showed that the mean disease index of Fusarium wilt was 29.4 and 4.4 respectively, and the bacteriostatic effects were 39.9% and 85.8% compared with the control orchard soil respectively. The 86-1 cotton roots in the above-mentioned plots within 5cm soil samples, the average disease index 19.5 potplant blight, antibacterial effect of 60.5%. Even for more than 10 years of wilt nursery soil samples, the pathology was 27.2 and 6.8, respectively, antibacterial effect of 43.9% and 76.2%. 1990-1991 in Liaoning Province by the work of the test results, even for more than 10 years Fusarium wilt field antibacterial effect of 56.2%, continuous cropping 5 years antibacterial effect of 17.6%. The above results show that the main reason for the decline of Fusarium is the production and existence of Fusarium oxysporum f. The results showed that the amount of Fusarium bacteria in soil samples decreased by 48.6% -59.2% compared with that in orchard. Bacteriostatic soil after autoclaving bacteriostatic effect completely disappeared, initially proved that the antibacterial factor may be mainly biological factors.