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目的建立滚环扩增技术检测沙眼衣原体L型(L1,L2,L3)的方法。方法使用沙眼衣原体Omp1靶基因。设计衣原体L1,L2,L3型特异性锁式探针。使用衣原体Omp基因通用引物扩增标本,L1,L2,L3型特异性锁式探针与扩增的靶基因杂交结合形成环化单链分子,加入引物在Corbett RotorGeneTM 6000扩增仪滚动扩增环化单链分子,观察结果。结果滚环扩增技术检测上述3型衣原体,各型之间无交叉反应。结论滚环扩增作为一种新的扩增技术能检测L型衣原体。
Objective To establish a method for detecting L type (L1, L2, L3) of Chlamydia trachomatis by rolling circle amplification. Methods Chlamydia trachomatis Omp1 target gene was used. Design chlamydia L1, L2, L3-specific lock-type probe. The common primer of Omp gene of chlamydia was used to amplify the sample. The specific lock probes L1, L2 and L3 were hybridized with the amplified target gene to form the cyclized single-stranded molecule. The primer was added into a Corbett RotorGeneTM 6000 Amplifier Single-stranded molecules, the results. Results Rolling circle amplification assay for the above type 3 Chlamydia, no cross-reaction between the various types. Conclusion Rolled-loop amplification can be used as a new amplification technique to detect L-type Chlamydia.