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目的 探讨谷氨酸对视网膜内层神经细胞 (inner stratum retinal neurons,ISRN)的影响及其毒性作用的量效关系。 方法 将 30只新生小鼠神经层视网膜组织块均匀接种于 2块 2 4孔培养板 (4 8孔 ) ,将其分成正常对照组、谷氨酸作用 2 4h组和正常培养 18h后 ,谷氨酸继续作用 6 h组。培养 2 4h后 ,将视网膜组织块常规进行切片 ,HE染色 ,油镜下观察视网膜神经节细胞层 (retinal ganglion cells,RGCs)和内核层 (inner nuclear layer,INL)的细胞形态 ,并分别计算细胞形态正常的 RGCs数和 INL 细胞数。 结果 视网膜组织正常培养 2 4h后 ,ISRN(RGCs和 INL细胞 )中可见少数细胞胞核固缩和坏死。谷氨酸作用 2 4h组 :当谷氨酸浓度为 2、4mmol时 ,ISRN的细胞形态与对照组之间差异无显著性意义 (P>0 .0 5 ) ;谷氨酸浓度≥ 6 mmol,视网膜内层形态正常的神经细胞数量明显较对照组减少 (P<0 .0 5 ) ,且随着谷氨酸浓度的增加 ,其数量逐渐减少。谷氨酸后 6 h作用组 :谷氨酸浓度为 6 mmol时 ,INL中形态正常的细胞数量明显较对照组减少 (P<0 .0 5 ) ,而 RGCs层中形态正常的细胞数量与对照组之间差异无显著性的意义 (P>0 .0 5 ) ;谷氨酸浓度≥ 8mmol时 ,RGCs层和 INL中形态正常的细胞数量均较对照组明显减少 (P<0 .0 5 )。?
Objective To investigate the effect of glutamate on the inner stratum retinal neurons (ISRN) and the dose-response relationship of its toxicity. Methods Thirty newborn mouse retinas of retina were evenly inoculated into two 24-well plates (48 holes) and divided into normal control group, glutamate-treated group for 24 hours and normal cultured group for 18 hours. Acid continue 6 h group. After cultured for 24 hours, the retinal tissue pieces were routinely sectioned and stained with HE. The morphology of retinal ganglion cells (RGCs) and inner nuclear layer (INL) were observed under an oil microscope. The number of normal RGCs and INL cells. RESULTS: After retinal tissue culture for 24 hours, a few nuclei were found in ISRN (RGCs and INL cells). Glutamate group24h group: When the concentration of glutamate was2.4mmol, there was no significant difference between ISRN cell morphology and the control group (P> 0.05); glutamate concentration≥6mmol, The number of normal retina inner neurons decreased significantly compared with the control group (P <0.05), and gradually decreased with the increase of glutamate concentration. 6 h after glutamate group: the concentration of 6 mmol glutamate, INL cells in normal morphology was significantly reduced compared with the control group (P <0. 05), while the number of RGCs layer normal cells and control There was no significant difference between the two groups (P> 0.05); when the concentration of glutamate was higher than 8mmol, the number of normal cells in RGCs layer and INL group were significantly decreased compared with the control group (P <0.05) . ?