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生长激素释放因子(GRF)于1982年发现后,关于人工合成GRF的药物动力学作用已有很多报告,而未见关于内源性GRF释放的报告。为说明GRF在临床医学中的病理生理学作用,作者使用一种敏感,特异的放免法,研究正常人及病人免疫反应性GRF的释放模式。人工合成hGRF(1—44)NH_2以氯胺T方法碘化,以1×10Cm羟甲基纤维素柱(CM-23)提纯。该研究所使用的抗血清,已证实可识别N端和hGRF中部。采用合成的[Met(0)]~(27)—hGRF(1—44)NH_2为标准制剂,测定的敏感性为每管4Pg。为清除引起非特异性干扰的物质,以冷丙酮/冰醋酸提取血浆,测定前再用石油醚提取。
After the discovery of growth hormone releasing factor (GRF) in 1982, there have been many reports on the pharmacokinetic effects of synthetic GRF, but no reports on the release of endogenous GRF have been reported. To illustrate the pathophysiological role of GRF in clinical medicine, the authors used a sensitive and specific radioimmunoassay to study the release patterns of immune-reactive GRF in normal individuals and patients. Synthetic hGRF (1-44) NH_2 was chloramine T-iodinated and purified with 1 × 10Cm hydroxymethyl cellulose column (CM-23). The antisera used in this study has been shown to recognize the N-terminus and the middle of hGRF. Synthetic [Met (0)] ~ (27) -hGRF (1-44) NH_2 was used as a standard formulation to measure the sensitivity of 4Pg per tube. To remove substances that cause non-specific interference, extract the plasma with cold acetone / glacial acetic acid and extract with petroleum ether before assaying.