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用已设计的一对特异引物和建立的双温度点聚合酶链式反应（ＰＣＲ）技术，分别从我国安徽、福建、海南、四川及云南五省疟区采集的间日疟患者血样ＤＮＡ抽提物中，扩增出长约６４０个碱基对（ｂｐ）的ＤＮＡ片段，经限制性酶切鉴定，证实为间日疟原虫小亚单位核糖体核糖核酸基因（ＳＳＵｒＤＮＡ）目的片段。将其分别与载体Ｍ１３ｍｐ１８和Ｍ１３ｍｐ１９连接、克隆，以双脱氧末端终止法测序，并分析比较。结果表明，同一地区二份样本序列完全相同，国内间日疟原虫地理株间及其与已报道的国外虫株克隆间，该序列显示出高度的同源性，但亦存在由个别碱基置换，插入或缺失所表现出的差异。
DNA was extracted from blood samples of malaria patients collected from malaria areas in five provinces of Anhui, Fujian, Hainan, Sichuan and Yunnan in China respectively using a pair of specific primers designed and double temperature point polymerase chain reaction (PCR) A DNA fragment of about 640 base pairs (bp) was amplified and identified by restriction enzyme analysis. The result showed that it was a target fragment of the small subunit ribosomal RNA gene (SSUrDNA) of Plasmodium vivax. They were cloned into vectors M13mp18 and M13mp19 respectively, cloned, sequenced by dideoxy terminator method and analyzed and compared. The results showed that the two regions of the same sample sequence exactly the same between the domestic strains of Plasmodium vivax and its insect strains have been reported abroad, the sequence showed a high degree of homology, but there are also by individual base replacement , Insertions, or deletions.