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目的： 探讨比较阿霉素（ ADM）与 43℃加热单独或合并处理人肝癌细胞 7721敏感株（简称 7721细胞）和人肝癌细胞 7721/Adm耐药株（简称 7721/Adm细胞）的细胞毒作用。方法：以体外培养的人肝癌细胞 7721敏感株和已经建立的人肝癌细胞 7721/Adm耐药株为研究对象，采用水浴加温法 ,体外细胞毒试验（ MTT法） ,观察 ADM与加热处理对细胞生长抑制的影响；采用流式细胞技术检测热处理对 7721细胞和 7721/Adm细胞胞内阿霉素浓度的影响。结果： (1)热处理可以明显提高两种细胞对阿霉素的敏感性： 7721细胞、 7721/Adm细胞经阿霉素及 43.5℃热处理，其细胞存活率分别下降 35.2％（ 30 min）、 24.8％（ 60 min）和 29.4％（ 30 min）、 22.8％（ 60 min）； (2)流式细胞仪检测显示，热处理可明显提高这两种细胞尤其是 7721/Adm细胞内的阿霉素浓度： 7721细胞组提高 30.8％， HCC 7721/Adm组提高 51％。结论：热处理可以显著提高人肝癌细胞 7721敏感株和人肝癌细胞 7721/Adm耐药株对阿霉素的敏感性。
OBJECTIVE: To investigate the effects of adriamycin (ADM) and heating at 43°C on human hepatocellular carcinoma 7721-sensitive strain (referred to as 7721 cells) and human hepatoma cells 7721/Adm-resistant strain (referred to as 7721/Adm cells) alone or in combination. Poisonous effect. METHODS: Human hepatocellular carcinoma 7721-sensitive strain and in vitro established human hepatoma cell 7721/Adm resistant strain were studied. Water bath heating method and in vitro cytotoxicity assay (MTT method) were used to observe ADM and heating. The effect of treatment on cell growth inhibition was examined; the effect of heat treatment on the intracellular adriamycin concentration of 7721 cells and 7721/Adm cells was examined by flow cytometry. RESULTS: (1) Heat treatment significantly increased the sensitivity of the two cells to adriamycin: 7721 cells, 7721/Adm cells were treated with adriamycin and heat treated at 43.5°C. The cell viability was decreased by 35.2% (30 min) and 24.8 respectively. % (60 min) and 29.4% (30 min), 22.8% (60 min); (2) Flow cytometry showed that heat treatment significantly increased doxorubicin concentration in both cells, especially 7721/Adm cells. : 7721 cell group increased 30.8%, HCC 7721/Adm group increased by 51%. Conclusion: Heat treatment can significantly improve the sensitivity of human hepatocellular carcinoma 7721-sensitive strain and human hepatoma cell 77021/Adm-resistant strain to doxorubicin.