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通过组织学和免疫组织化学法对长蛇鮈肠组织形态学及ghrelin在其内的定位进行初步研究。结果显示,长蛇鮈肠壁组织由内向外由粘膜层、粘膜下层、肌层、浆膜层组成。中肠粘膜层厚度及由粘膜层形成的肠皱褶长度均显著大于前肠和后肠(P<0.05);前肠和中肠粘膜下层、肌肉层和浆膜层厚度显著高于后肠(P<0.05),但这3层的厚度在前肠和中肠不存在显著性差异(P>0.05)。ghrelin在长蛇鮈的前肠、中肠与后肠的粘膜层均无分布,但在粘膜下层均有分布,且前肠和中肠的粘膜下层呈强阳性反应,后肠粘膜下层阳性反应较弱;长蛇鮈前、中、后肠各区段肌肉层和浆膜层均见ghrelin阳性反应,但无明显差异。浆膜层ghrelin阳性反应要比肌肉层明显。因此,根据长蛇鮈肠道组织学结构差异,可将其明显的分为前肠、中肠和后肠;由ghrelin在肠道的分布规律发现其在长蛇鮈的摄食和食物消化吸收的内分泌调控中起重要调控作用。
Histopathology and immunohistochemistry were used to study the histological morphology and ghrelin histology of C. elegans. The results showed that the serpentine intestinal wall tissue from the inside out by the mucosa, submucosa, muscularis, serosal composition. The mucosal thickness of the midgut and the length of gut folds formed by the mucosa were significantly greater than those of the foregut and the hindgut (P <0.05). The thickness of the mucosa, muscular and serosa of the foregut and midgut were significantly higher than that of the hindgut P <0.05). However, there was no significant difference in the thickness of the three layers between the foregut and the midgut (P> 0.05). ghrelin had no distribution in the mucosa of the foregut, midgut and hindgut of the snakehead snakehead, but distributed in the submucosa, and the submucosa of the foregut and the midgut showed a strong positive reaction. The positivity of the hindgut mucosa was lower than that of the ghrelin Weak; snakehead snakehead, middle and hindgut sections of the muscle layer and serosa were ghrelin positive reaction, but no significant difference. Serosal ghrelin positive reaction than muscle layer obvious. Therefore, according to histological differences in the structure of gut snake intestine, it can be clearly divided into the foregut, the midgut and the hindgut; ghrelin in the gut distribution pattern found in the snakehead snakehead food intake and food digestion and absorption Endocrine regulation plays an important regulatory role.