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目的观察兔蛛网膜下腔出血(SAH)后基底动脉的血小板源性生长因子受体-β(PDGFR-β)的表达变化规律,从侧面反映血小板源性生长因子(PDGF)-BB在SAH后脑血管痉挛(CVS)的作用。方法采用枕大池二次注血法建立稳定的兔CVS模型,应用HE染色、透射电镜、免疫组化技术等方法,观察兔基底动脉的超微结构变化及PDGFR-β的表达时间窗,并通过测量管径的变化来判断CVS的严重程度。结果电镜结果提示SAH组的基底动脉部分内皮细胞脱落,紧密联接打开,内皮间隙扩大;内弹力膜扭曲、断裂;平滑肌细胞胞浆空泡状,排列紊乱;外膜可见大量中性粒细胞、单核细胞浸润;管腔狭窄呈急性期收缩和迟发性痉挛双相改变;免疫组化结果提示对照组的PDGFR-β无阳性表达,SAH组的PDGFR-β表达升高,在第5、7天时最为明显(P<0.01),第10天后表达稍下降,但仍高于对照(P<0.05)。结论 PDGFR-β在SAH后CVS中基底动脉的表达呈现一定的时序性,提示PDGF-BB在迟发性脑血管痉挛的发生发展中起作用。
Objective To observe the changes of platelet-derived growth factor receptor-β (PDGFR-β) in basilar artery after subarachnoid hemorrhage (SAH) in rabbits, and to observe the expression of platelet-derived growth factor (PDGFR- Vasospasm (CVS) effect. Methods The stable rabbit model of CVS was established by double injection of occipital cistern and the ultrastructure of basilar artery in rabbits and the expression time window of PDGFR-β were observed by HE staining, transmission electron microscopy and immunohistochemistry. Measure changes in diameter to determine the severity of CVS. Results Electron microscopy showed that part of endothelial cells in basilar artery of SAH group were exfoliated and closely connected to open, and the endothelial gap was enlarged. The inner elastic membrane was distorted and ruptured. The cytoplasm vacuolization of smooth muscle cells was disordered. A large number of neutrophils were observed in the outer membrane The results of immunohistochemistry showed that there was no positive expression of PDGFR-β in the control group, while the expression of PDGFR-β in SAH group was increased. In the 5th, 7th and 7th week, (P <0.01). After 10 days, the expression was slightly decreased, but still higher than the control (P <0.05). Conclusions The expression of PDGFR-β in basal ganglia in CVS after SAH shows a certain time sequence, suggesting PDGF-BB plays a role in the development of delayed cerebral vasospasm.