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目的白介素(IL)-18是参与溃疡性结肠炎(UC)发病的一个重要细胞因子。本研究检测中国汉族UC患者中IL-18基因的单核苷酸多态性(SNPs),分析其在UC发病中的意义。方法在102例UC患者和128例正常对照中,用PCR扩增和直接测序法检测IL-18基因中的2个SNP(-656G/T、-607C/A)的基因分型。结果在UC患者中,SNP位点-656G/T、-607C/A等位基因频率及它们的基因型频率与健康对照组没有显著性差异。结论IL-18基因上-656G/T、-607C/A SNPs及单倍体型与中国汉族UC患者之间无相关性。
Interleukin (IL) -18 is an important cytokine involved in the pathogenesis of ulcerative colitis (UC). The aim of this study was to examine single nucleotide polymorphisms (SNPs) of IL-18 gene in Chinese Han patients with UC and to analyze its significance in the pathogenesis of UC. Methods The genotypes of two SNPs (-656G / T, -607C / A) in IL-18 gene were detected by PCR amplification and direct sequencing in 102 UC patients and 128 normal controls. Results The frequencies of -656G / T and -607C / A alleles at SNP loci and their genotype frequencies in UC patients were not significantly different from those in healthy controls. Conclusion There is no correlation between -656G / T, -607C / A SNPs and haplotype in IL-18 gene and Chinese Han patients with UC.