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目的:系统研究不同训练方案运动后静息状态ATP含量及ATP酶活性的特异性、适应性变化,以评估不同训练方案的训练效果;为尝试寻找更有效的专项训练方法提供一些科学依据和参考。材料与方法:将86只SPF级雄性SD大鼠随机分为对照组(S组)、耐力组(E组)、间歇组(I组)、“耐力+间歇”组(EI组)及“间歇+耐力”组(IE组),于4周、8周运动结束48 h后取样、提取左侧股直肌,测定ATP含量及ATP酶活性。结果:EI8组、I8组和IE8组ATP含量显著高于S8组(P<0.05);EI8组ATP含量极显著高于EI4组(P<0.01);IE8组ATP含量极显著高于同周其他3个运动组、IE4组(P<0.01)。I8组和IE8组Ca2+-ATP酶活性显著高于S8组(P<0.05);I8组和IE8组Mg2+-ATP酶活性极显著高于S8组和EI8组(P<0.01);I8组Mg2+-ATP酶活性还极显著高于E8组(P<0.01);EI8组Mg2+-ATP酶活性显著低于EI4组(P<0.05)。结论:4种训练方案中“间歇+耐力”训练对提高股直肌静息ATP含量效果最佳;间歇运动和“间歇+耐力”运动均显著提高股直肌静息Ca2+-ATP酶和Mg2+-ATP酶活性,显著提高骨骼肌利用ATP的速率,加快肌肉收缩速率,提高肌肉工作张力。
OBJECTIVE: To systematically study the specificity and adaptive changes of ATP content and ATPase activity in resting state after exercise training to evaluate the training effect of different training programs, and to provide some scientific evidence and reference for trying out more effective training methods . MATERIALS AND METHODS: Totally 86 SPF male Sprague-Dawley rats were randomly divided into control group (S group), endurance group (E group), intermittent group (I group), endurance + intermittent group The rats in “intermittent + endurance” group (IE group) were sampled at 4 weeks and 8 weeks after the end of exercise for 48 hours. The left rectus femoris muscle was extracted and the ATP content and ATPase activity were measured. Results: The content of ATP in EI8 group, I8 group and IE8 group was significantly higher than that in S8 group (P <0.05). The content of ATP in EI8 group was significantly higher than that in EI4 group (P <0.01) 3 sports group, IE4 group (P <0.01). The activities of Ca2 + -ATPase in I8 and IE8 groups were significantly higher than that in S8 group (P <0.05). The activities of Mg2 + -ATPase in I8 and IE8 groups were significantly higher than those in S8 and EI8 groups (P <0.01) (P <0.01). The activity of Mg2 + -ATPase in EI8 group was significantly lower than that in EI4 group (P <0.05). CONCLUSION: The training of “intermittent + endurance” can improve the resting ATP content of rectus femoris muscle. Intermittent exercise and “intermittent + endurance” exercise significantly increase resting Ca2 + -ATP Enzymes and Mg2 + -ATPase activity, significantly increase the rate of skeletal muscle use of ATP, accelerate muscle contraction rate, improve muscle working tension.