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目的：从抗变形链球菌ＳＡⅠ／Ⅱ单抗杂交瘤细胞系２Ｂ１２Ｆ６中扩增克隆重链可变区基因。方法：采用ＰＣＲ技术和基因工程技术，扩增杂交瘤细胞系２Ｂ１２Ｆ６的重链可变区基因并克隆入载体ｐＵＣ１８，Ｓａｎｇｅｒ双脱氧链末端终止法测定核苷酸序列。结果：重链可变区基因全长３６０ｂｐ，编码１１８个氨基酸，其框架区与发表的小鼠重链可变区基因序列有７０％的同源性，符合鼠重链可变区基因的结构特征。结论：抗变形链球菌ＳＡⅠ／Ⅱ单抗杂交瘤细胞系２Ｂ１２Ｆ６重链可变区基因的获得是构建抗变形链球菌ＳＡⅠ／Ⅱ基因工程抗体的基础。
OBJECTIVE: To amplify the cloned heavy chain variable region genes from the anti-Streptococcus mutans SAⅠ / Ⅱ hybridoma cell line 2B12F6. Methods: The heavy chain variable region of hybridoma cell line 2B12F6 was amplified by PCR and gene engineering. The heavy chain variable region gene was amplified and cloned into vector pUC18. The nucleotide sequence was determined by Sanger dideoxy chain termination method. RESULTS: The heavy chain variable region gene was 360bp in length and encoded a polypeptide of 118 amino acids. The heavy chain variable region gene has 70% homology with the published mouse heavy chain variable region gene sequence, which matches the structure of the mouse heavy chain variable region gene feature. Conclusion: The obtainment of 2B12F6 heavy chain variable region gene of Streptococcus mutans SAⅠ / Ⅱ hybridoma cell line is the basis of constructing anti-Streptococcus SA Ⅰ / Ⅱ gene engineering antibody.