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为进一步探明polCMS育性恢复基因作用的分子机理,利用数字基因表达谱技术,选用不育系与恢复系杂交的F2代分离群体,对大白菜polCMS育性恢复相关基因的差异表达进行了分析,并选取部分基因进行了实时荧光定量PCR验证。共有2826个基因差异表达,其中441个上调表达,2385个下调表达。GO功能注释表明,差异表达基因显著富集的细胞位置为细胞质、细胞器及大分子复合物等位置,细胞器包括线粒体、叶绿体及质体等,分子功能主要为核酸外切酶的活性,参与的生物过程是花粉壁的形成和组装。与polCMS显著相关的通路主要是核糖体、糖和氨基酸代谢、核苷酸切除和修复、RNA降解等通路。表达谱和RT-PCR结果表明恢复基因主要通过下调表达调控育性恢复,有4个差异表达基因与育性恢复密切相关。
In order to further investigate the molecular mechanism of polCMS fertility restorer gene function, the differential expression of polCMS fertility-related genes in Chinese cabbage was analyzed by using digital gene expression profiling technique. F2 segregating populations crossed with CMS lines and restorer lines were selected , And selected some genes for real-time fluorescence quantitative PCR validation. A total of 2826 genes were differentially expressed, of which 441 were up-regulated and 2385 down-regulated. GO functional annotation indicates that the differentially expressed genes are significantly enriched in cellular locations such as cytoplasm, organelles and macromolecular complexes, including organelles, mitochondria, chloroplasts and plastids. The molecular functions are mainly exonuclease activity, and the involved organisms The process is the formation and assembly of pollen walls. Pathways that are significantly associated with polCMS are primarily ribosomal, sugar and amino acid metabolism, nucleotide excision and repair, RNA degradation and other pathways. Expression profiling and RT-PCR results showed that restorer gene mainly regulates fertility restoration through down-regulation, and four differentially expressed genes are closely related to fertility restoration.